Toxicology and Pharmacology

Biography

Abdul Mannan H Fateh is currently pursuing his PhD study (last year) at Pharmacology Department, Faculty of Medicine, and University of Malaya, Malaysia. He has completed his Master degree MSC in Toxicology, department of Medical Elementology and Toxicology, Hamdard University, New Delhi, India. He has worked in Medical College from 2008 – 2013 as a Lecturer of Toxicology. He has published three research papers in peer-reviewed journals.

Abstract

Verbena officinalis is traditionally used for various reproductive problems in women. However, the data of its genotoxic and mutagenic potential effects have not been assessed. Two experiments were conducted to investigate the mutagenic (in vitro) and genotoxic (in vivo) potential utilizing OECD guidelines. In vitro Ames assay was conducted using salmonella/microsome test (TA100, TA98, TA1535, TA97a, and Escherichia coli WP2 uvrA (pKM101)) with and without the metabolic activation mixture. For the in vivo micronucleus of the bone marrow study, female and male SD rats were administered a daily single oral dose of either 500, 1000 or 2000 mg/kg of the extract of V. officinalis over three days. The positive control group was injected IP with a single dose of cyclophosphamide (50 mg/kg), while the negative control received 5 ml/kg of distilled water orally. Ames test findings showed an increase mutagenic effect in a dose dependent pattern of V. officinalis extract against TA98 and TA100 strains. The micronucleus assay showed no genotoxicity effect for V. officinalis on both male and female rats, while cyclophosphamide significantly increased in the micronucleus polychromatic erythrocytes as compared to untreated rats. V. officinalis aqueous extract has demonstrated a mutagenic activity against TA100 and TA98 strains, while no clastogenic and myelotoxic effects of V. officinalis in rats. Therefore, women should be aware that traditional herbs may have toxicity or adverse effects and need to consult doctors before using any medicinal plants, particularly during pregnancy.